SPOP Knockout cell line (Hep G2)

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location： Home > Products > KO Cell Lines > SPOP Knockout cell line (Hep G2)

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SPOP Knockout cell line (Hep G2) Order

SPOP Knockout cell line (Hep G2)
Catalog#
YKO-HT3387
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
SPOP Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
SPOP Knockout cell line (Hep G2)
Species
Human
Gene Name
SPOP
Gene ID
8405
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 3 (based on transcript SPOP-206)
Validation: PCR+Sanger Sequencing
SPOP Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the SPOP gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, SPOP gene knockout SPOP Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
SPOP Knockout cell line (Hep G2)
SPOP Knockout cell line (Hep G2)
Catalog#
YKO-HT3387
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • SPOP Knockout cell line(5637)

    SPOP Knockout cell line(5637)
      inquiry   More details

    Organism: Human
    Gene Name: SPOP
    Gene ID: 8405
    Size: 1*10^6
    Catalog#: YKO-H520
    YKO-H520(5637)
  • SPOP Knockout cell line (HeLa)

    SPOP Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: SPOP
    Gene ID: 8405
    Size: 1*10^6
    Catalog#: YKO-HS4127
    YKO-HS4127(HeLa)
  • SPOP Knockout cell line (HCT 116)

    SPOP Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: SPOP
    Gene ID: 8405
    Size: 1*10^6
    Catalog#: YKO-HS3004
    YKO-HS3004(HCT 116)

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Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • SPOP Knockout cell line(5637)

    SPOP Knockout cell line(5637)

    Organism: Human
    Gene Name: SPOP
    Gene ID: 8405
    Size: 1*10^6
    Catalog#: YKO-H520
      inquiry   More details
  • SPOP Knockout cell line (HeLa)

    SPOP Knockout cell line (HeLa)

    Organism: Human
    Gene Name: SPOP
    Gene ID: 8405
    Size: 1*10^6
    Catalog#: YKO-HS4127
      inquiry   More details
  • SPOP Knockout cell line (HCT 116)

    SPOP Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: SPOP
    Gene ID: 8405
    Size: 1*10^6
    Catalog#: YKO-HS3004
      inquiry   More details

SPOP Knockout cell line (Hep G2) Order

SPOP Knockout cell line (Hep G2)
Catalog#
YKO-HT3387
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
SPOP Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
SPOP Knockout cell line (Hep G2)
Species
Human
Gene Name
SPOP
Gene ID
8405
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 3 (based on transcript SPOP-206)
Validation: PCR+Sanger Sequencing
SPOP Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the SPOP gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, SPOP gene knockout SPOP Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
SPOP Knockout cell line (Hep G2)
SPOP Knockout cell line (Hep G2)
Catalog#
YKO-HT3387
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • SPOP Knockout cell line(5637)

    SPOP Knockout cell line(5637)
      inquiry   More details

    Organism: Human
    Gene Name: SPOP
    Gene ID: 8405
    Size: 1*10^6
    Catalog#: YKO-H520
    YKO-H520(5637)
  • SPOP Knockout cell line (HeLa)

    SPOP Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: SPOP
    Gene ID: 8405
    Size: 1*10^6
    Catalog#: YKO-HS4127
    YKO-HS4127(HeLa)
  • SPOP Knockout cell line (HCT 116)

    SPOP Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: SPOP
    Gene ID: 8405
    Size: 1*10^6
    Catalog#: YKO-HS3004
    YKO-HS3004(HCT 116)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • SPOP Knockout cell line(5637)

    SPOP Knockout cell line(5637)

    Organism: Human
    Gene Name: SPOP
    Gene ID: 8405
    Size: 1*10^6
    Catalog#: YKO-H520
      inquiry   More details
  • SPOP Knockout cell line (HeLa)

    SPOP Knockout cell line (HeLa)

    Organism: Human
    Gene Name: SPOP
    Gene ID: 8405
    Size: 1*10^6
    Catalog#: YKO-HS4127
      inquiry   More details
  • SPOP Knockout cell line (HCT 116)

    SPOP Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: SPOP
    Gene ID: 8405
    Size: 1*10^6
    Catalog#: YKO-HS3004
      inquiry   More details

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